More than 90% of the events are assigned to two clusters, one on the '+' strand and one on the '-' strand. These '+' and '-' strand clusters also have similar read counts. Why does this happen?
I used regtools with the -s RF option, because using -s XS resulted in empty clusters.
Below are two examples:
chr1:111137586:111139501:clu_100_- 8 8 24 14 25
chr1:111137586:111140038:clu_100_- 28 0 14 31 0
chr1:111137586:111139501:clu_309_+ 6 10 12 11 20
chr1:111137586:111140038:clu_309_+ 26 0 16 38 0
chr1:114727090:114730258:clu_105_- 136 598 57 75 627
chr1:114727090:114732604:clu_105_- 15 47 0 1 56
chr1:114730648:114732604:clu_105_- 104 494 40 80 543
chr1:114727090:114730258:clu_314_+ 111 568 46 78 556
chr1:114727090:114732604:clu_314_+ 5 51 1 0 39
chr1:114730648:114732604:clu_314_+ 76 504 40 48 518
More than 90% of the events are assigned to two clusters, one on the '+' strand and one on the '-' strand. These '+' and '-' strand clusters also have similar read counts. Why does this happen?
I used regtools with the -s RF option, because using -s XS resulted in empty clusters.
Below are two examples:
chr1:111137586:111139501:clu_100_- 8 8 24 14 25
chr1:111137586:111140038:clu_100_- 28 0 14 31 0
chr1:111137586:111139501:clu_309_+ 6 10 12 11 20
chr1:111137586:111140038:clu_309_+ 26 0 16 38 0
chr1:114727090:114730258:clu_105_- 136 598 57 75 627
chr1:114727090:114732604:clu_105_- 15 47 0 1 56
chr1:114730648:114732604:clu_105_- 104 494 40 80 543
chr1:114727090:114730258:clu_314_+ 111 568 46 78 556
chr1:114727090:114732604:clu_314_+ 5 51 1 0 39
chr1:114730648:114732604:clu_314_+ 76 504 40 48 518