run_leafviz.R | Splicing event visualization table and Gene-level visualization errors

[yenon118]

Hi,

I could generate the RData using prepare_results.R script like the command below. After that, I used the RData on LeafViz. However, when I clicked on some of the genes, the table in the Splicing event visualization could not be loaded and had the "Error: duplicate 'row.names' are not allowed" error message. At the Gene-level visualization, I also received "Error: ength(unique(exons$chr)) == 1 is not TRUE" error message. Does anybody know what is the possible cause of this error and the potential solutions to the issue?

The command:

Rscript ../../../../tools/leafcutter/leafviz/prepare_results.R \
--meta_data_file ../data/2023_06_15_run_Leafcutter_ds/group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP.txt \
--code 2023_10_16_Leafcutter_group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP \
-o ../output/2023_10_16_run_Leafcutter_prepare_results/group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP/2023_10_16_Leafcutter_group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP.RData \
../output/2023_06_10_run_Intron_Clustering/testYRIvsEU_perind_numers.counts.gz \
../output/2023_06_16_run_Leafcutter_ds/group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP/2023_06_16_group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP__cluster_significance.txt \
../output/2023_06_16_run_Leafcutter_ds/group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP/2023_06_16_group_file__AR_A_M_1hr_IP__WT_A_M_1hr_IP__effect_sizes.txt \
../output/2023_09_05_run_Leafcutter_gtf2leafcutter/2023_09_05_Mus_musculus.GRCm39.107

LeafViz output:

Error messages:

Thank you.